Reflectoquant® Lactic Acid, Catalog No. 16127/1
1. Method
Lactic acid (lactate) is oxidized by nicotinamide adenine dinucleotide (NAD) under the catalytic
effect of lactate dehydrogenase to form pyruvate. In the presence of diaphorase, the NADH
formed in the process reduces a tetrazolium salt to form a blue formazan, the concentration of which
is determined reflectometrically.
2. Measuring Range and Number of Determinations
Measuring Range |
Number of Determinations |
1.0 - 60.0mg/l lactic acid |
50 |
3. Applications
This test measures total lactic acid (sum of D- and L-lactic acid).
Sample Material:
Beverages, e.g., wine, beer, and fruit/vegetable juice after appropriate dilution (see section
6). Yogurt after appropriate dilution (see section 6). Food after appropriate sample
pretreatment.
4. Influence of Foreign Substances
After appropriate dilution or sample pretreatment, the determination is not interfered with by
the concentrations of foreign substances usually present in beverages and food.
5. Reagents and Auxiliaries
The analytical test strips are stable at least up to the date stated on the pack when
stored in the closed tube at +2 to +8°C. |
Package Contents:
Tube containing 50 analytical test strips 1 Bar code strip
Other Reagents and Accessories:
L(+)-Lactic acid monolithium salt, Cat. No. 1.00377, Merck KGaA, Darmstadt, Germany
6. Preparation
Liquid samples can be analyzed after appropriate sample pretreatment, e.g., after dilution
with distilled water (applications available on request). In the case of dilutions of
1:50 or higher (e.g., 1ml of sample + 49ml of water), interferences due to intrinsic
colorations can be neglected even in very intensively colored samples.
To exclude interferences due to foreign substances, at least the following dilutions are
necessary:
Wines: |
1:100 |
Sake: |
1:50 |
Beers: |
1:50 |
Yogurt: |
1:100 |
Fruit and Vegetable Juices: |
1:50 |
7. Procedure
Observe the instructions for the reflectometer. The following applies to the Lactic Acid Test:
Measurement Procedure A.
Stored reaction time: 300 sec.
Press the START button of the reflectometer and - this is imperative -
at the same time immerse both reaction zones of the analytical test strip in the
measurement sample for approximately 2 seconds.
Allow excess liquid to run off via the long edge of the strip onto
an absorbent paper towel.
Approximately 10 seconds before the end of the reaction time,
insert the test strip all the way
into the strip adapter with the reaction zones facing the display.
Read off the result from the display in mg/l lactic acid.
The result is automatically stored. |
Notes on the Measurement:
- The two reaction zones change color differently.
- Concerning the result of the analysis, the dilution (see section 6) must be taken into
account:
Result of analysis = measurement value x dilution factor
8. Method Control
To check analytical test strips, equipment and
procedure:
Dissolve 1.0766g of L(+)-lactic acid monolithium salt in distilled water, make up to 1000ml
with distilled water, and mix. Lactic acid content 1000mg/l.
Dilute this standard solution with distilled water to 25mg/l lactic acid and analyze as
described in section 7.
9. Notes
- Reclose the tube containing the test strips immediately after use.
- Do not touch the reaction zones with the fingers, since hand perspiration contains
lactic acid.
- At the end of each workday, cleanse the strip adapter thoroughly with water or ethanol.
Purchase Lactic Acid Test Strips 1-60ppm Reflectoquant 1 PK *Requires RQflex 2 EMD# 16950-1
Gallade Part#: 16127-1
Mfr Part#: 16127-1
Brand: EMD Chemicals EM Science
Container: 1 PK of 50 Strips
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